Wyniki konkursu:
2019.09.19 raport komisji konkursowej – adiunkt naukowy – Ł. Markiewicz W ramach przeprowadzonego postępowania Komisja Konkursowa rekomendowała kandydaturę dr Łukasza Markiewicza na ww. stanowisko.
Opis:
Title of the position: Post-doc
Scientific discipline: Biology
Job type: Contract
Number of job offers: 1
Remuneration/Stipend amount/month: 7500 PLN gross/month
Position starts on: 01/12/2019
Maximum period of contract/stipend agreement: 36 months
Institution: Laboratory of Bioorganic Chemistry
Project leader: Paweł Sikorski
Project title: Towards understanding the cellular fate of exogenously delivered mRNA
Project Description: Exogenously delivered messenger RNA (mRNA) is a promising therapeutic agent with potential uses in cancer immunotherapies, gene therapies, and cell reprogramming. Despite clear advantage of using mRNA over DNA (e.g. mRNA does not integrate with the genome or has not to be delivered to the cell nucleus) several limitations must be overcome before mRNAs can be considered as therapeutic agents. Recently, much effort has been made either to improve cellular instability and to increase translational properties of in vitro transcribed (IVT) mRNA in complex cellular environment. However, there is also additional issue which is currently extensively studied, how immunogenic properties of IVT mRNA could modulate cell immune response. It is well known that exogenous RNA (e.g. RNA viruses) is recognized as non-self RNA and triggers cell immune defence. Key for this recognition are differences in methylation status of mRNA 5’ end. In higher Eukaryotes mRNAs bear cap-1 or cap-2 structure thus RNAs with triphosphate group or with cap-0 structure are recognized as non-self RNAs. Studies conducted so far on the cell immune response to exogenous RNA pointed out that immunogenic properties of IVT mRNA could be of great importance in its usage as therapeutic agent. Only recently, the possibility to produce IVT mRNA with non-immunogenic cap structures (cap-1 and cap-2) became available. The methods of synthesis of cap-1 and cap-2 analogues were established in Laboratory of Bioorganic Chemistry (CeNT UW) and by others (TriLink BioTechnologies). Therefore, we proposed to decipher the principles that govern recognition of IVT mRNA as non-self RNA by cell immune system. Moreover, we plan to study interplay between methylation status of mRNA 5’ end and factors responsible either for sensing or for degradation of exogenous RNA.
Key responsibilities include:
Characterization of the cellular fate of of exogenously delivered mRNA. Identification of genes involved in sensing of exogenously delivered mRNA, generation of stable mammalian cell line, subcellular localization studies of exogenously delivered RNAs, expression and purification of recombinant proteins.
Profile of candidates/requirements:
The successful post-doc candidate will have a PhD degree in biology or related discipline, experience in cell culture technique, confocal microscopy and molecular biology techniques, an additional advantage will be recombinant protein expression and purification. Scientific achievements confirming the qualifications will be required (at least two significant publication from the period of PhD studies or previous post-doc).
Required documents:
curriculum vitae including a description of prior research experience; a cover letter; a list of publications and conference presentations; at least two reference contacts (with phone numbers and e-mails)
We offer:
Stimulating and friendly work environment
Please submit the following documents to: Applications should be submitted by e-mail to Dr Pawel Sikorski (p.sikorski@cent.uw.edu.pl) no later than 31st of August 2019 with the term “Postdoc position SONATA” as email’s topic. Following an initial screening of the applications, selected candidates will be contacted by e-mail for further recruitment steps.
To allow us to process your data, please include the following statement in your application:
“I hereby consent to have my personal data processed by the University of Warsaw with its registered office at ul. KrakowskiePrzedmieście 26/28, 00-927 Warszawa for the purpose of carrying out a recruitment process and selecting an employee and concluding a contract for employment at the University of Warsaw. I have been informed of my rights and duties. I understand that provision of my personal data is voluntary.”
In accordance with Article 13 of REGULATION (EU) 2016/679 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 27 April 2016 on the protection of natural persons with regard to the processing of personal data and on the free movement of such data – general regulation on data protection (Official Journal of the EU L 119/1 of 4 May 2016) the University of Warsaw informs that:
- The Controller of your personal data is the University of Warsaw with its registered office at KrakowskiePrzedmieście 26/28, 00-927 Warszawa;
- The Controller has designated the Data Protection Officer who supervises the processing of personal data, and who can be contacted via the following e-mail address: iod@adm.uw.edu.pl;
- Your personal data will be processed for the purpose of carrying out a recruitment process and selecting an employee and concluding a contract for employment at the University of Warsaw;
- The provided data will be processed pursuant to Article 221 § 1 of the Act of 26 June 1974 Labour Code (uniformed text: Dz.U. of 2018, item 917) and your consent for processing of personal data;
- Provision of data in the scope stipulated in the Labour Code is mandatory, and the remaining data are processed according to your consent for processing of personal data;
- The data will not be shared with any external entities;
- The data will be stored until you withdraw your consent for processing of personal data;
- You have the right to access your personal data, to rectify, erase them, restrict their processing, object to processing, and to withdraw the consent at any time;
- You have the right to lodge a complaint to the President of the Office for the Protection of Personal Data.
Termin nadsyłania aplikacji: 31/08/2019
Słowa kluczowe: cap structure, RNA sensing, immunogenicity, in vitro transcription, RNA-seq, mRNP complexes
