Laboratory of Molecular Basis of Synaptic Plasticity

Neuronal plasticity is the ability of neurons to adapt permanent changes in response to environmental stimuli. This unique property of the nervous system allows for learning and memory formation.

At single-neuron level, plasticity is expressed by the activity of individual synapses, which in turn depends on the type of proteins locally synthesized at the synapse in response to stimulation. Some proteins present in dendrites and synapses are synthesized from mRNAs transported from the cell body in response to synaptic stimulation. Synaptic translation guarantees spatial and temporal control of protein synthesis, and a fast regulatory effect of the synthesized proteins on spine morphology and receptor signaling. This process has proven to be extremely important for the physiology of neurons. Its dysfunction leads to abnormalities observed in such disease syndromes as fragile X syndrome and autism, and is associated with abnormal spine morphology and connectivity.

The Laboratory of Molecular Basis of Synaptic Plasticity focuses on the identification of mRNAs and proteins undergoing local translation at the synapse in response to specific types of stimulation, and on gaining a better understanding of their synaptic functions. Our research will contribute to identification of key proteins important for synaptic plasticity. We use mouse models of human diseases, such as fragile X syndrome, which impair the process of local translation (FMR1 KO mice), as well as neuronal cell imaging techniques, biochemical and molecular methods, next-generation sequencing, and high resolution mass spectroscopy.

Magdalena Dziembowska, PhD
phone: +48 22 55 43721
room: 04.225

Selected publications:

Novel calcineurin A (PPP3CA) variant associated with epilepsy, constitutive enzyme activation and downregulation of protein expression
Rydzanicz, M., Wachowska, M., Cook, E. C., Lisowski, P., Kuźniewska, B., Szymańska, K., ... & Koppolu, A. (2018).
European Journal of Human Genetics, 27, 61-69.
Preparation of polysomal fractions from mouse brain synaptoneurosomes and analysis of polysomal-bound mRNAs
Kuzniewska, B., Chojnacka, M., Milek, J., & Dziembowska, M., (2018).
Journal of neuroscience methods, 293, 226-233.
The level of microRNA 21 is upregulated by rapamycin in serum of tuberous sclerosis complex patients and subependymal giant cell astrocytoma (SEGA)-derived cell cultures
Kuzniewska, B., Sadowski, K., Urbanska, K., Urbanska, M., Kotulska, K., Liszewska, E., ... & Dziembowska, M. (2018).
Folia Neuropathologica, 56(3), 167-174
Neurodevelopmental phenotype caused by a de novo PTPN4 single nucleotide variant disrupting protein localization in neuronal dendritic spines
Szczałuba, K., Chmielewska, J. J., Sokolowska, O., Rydzanicz, M., Szymańska, K., Feleszko, W., ... & Bargeł, E. (2018).
Clinical genetics
Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage
Chmielewska, J. J., Kuzniewska, B., Milek, J., Urbanska, K., & Dziembowska, M. (2018).
Molecular neurobiology, 1-19
Rapid, experience-dependent translation of neurogranin enables memory encoding
Jones, K., J., Templet, S., Zemoura, K., Kuzniewska, B., ... & Dziembowska, M. (2018)
Proceedings of the National Academy of Sciences, 115(15), E5805-E5814
A normal genetic variation modulates synaptic MMP‐9 protein levels and the severity of schizophrenia symptoms.
Lepeta, K., Purzycka, K.J., Pachulska‐Wieczorek, K., Mitjans, M., Begemann, M., Vafadari, B., Bijata, K., Adamiak, R.W., Ehrenreich, H., Dziembowska, M. and Kaczmarek, L., 2017
EMBO molecular medicine, p.e201707723.
miR-132 Regulates Dendritic Spine Structure by Direct Targeting of Matrix Metalloproteinase 9 mRNA
Jasińska, M., Miłek, J., Cymerman, I. A., Łęski, S., Kaczmarek, L., & Dziembowska, M.
Molecular Neurobiology, 53(7), 4701-4712.
miR-132 Regulates Dendritic Spine Structure by Direct Targeting of Matrix Metalloproteinase 9 mRNA
Jasińska, M., Miłek, J., Cymerman, I. A., Łęski, S., Kaczmarek, L., & Dziembowska, M. (2015)
Molecular neurobiology, 53(7), 4701-4712.
Structural plasticity of dendritic spinels requires GSK3α and GSK3β
Cymerman, I. A., Gozdz, A., Urbanska, M., Milek, J., Dziembowska, M., & Jaworski, J. (2015)
PloS one, 10(7), e0134018.
HAX-1: a novel p-body protein
Zayat, V., Balcerak, A., Korczynski, J., Trebinska, A., Wysocki, J., Sarnowska, E., ... & Grzybowska, E. A., Dziembowska M. (2015).
DNA and cell biology, 34(1), 43-54.