Profile
Leader
Team
Projects
Publications
Contact
Website |
Profile
Prof. Joanna Trylska
e-mail: joanna@cent.uw.edu.pl
phone: +48 22 55 43683
room: 04.03
ORCID: 0000-0002-1464-5323
SCOPUS ID: 6602930387
We conduct theoretical and experimental research aimed at discovering new antibiotics effective against bacteria. In our studies, we use methods from physics, chemistry, bioinformatics, molecular biology, microbiology, and molecular modeling. Using computational methods, we design antibacterial compounds; subsequently, we synthesize them, stabilize their tertiary structure, study their interaction with bacterial membranes, test their antibacterial activity, and ensure the absence of hemolytic properties. Another area of our research focuses on proteases, especially viral ones. We investigate how macromolecular crowding affects their dynamics and enzymatic activities. Our team comprises chemists, biophysicists, biologists, and bioinformaticians.
Our research areas include:
- The design, synthesis and testing antibacterial peptides
- The design, synthesis and testing of peptide nucleic acid (PNA) carriers to bacterial cells.
- The synthesis of antibiotic and peptide tandems to enhance their antibacterial activity.
- Investigation of how cellular crowding affects enzyme function and drug efficiency.
- The development of computational methods for molecular dynamics to study biomolecular interactions.
- The development of software and tools for the analysis of biomolecular dynamics.
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Team
Leader
Prof. Joanna Trylska
Postdocs / employees
Monika Wojciechowska, PhD
Piotr Maj, PhD
Mateusz Wdowiak, PhD
PhD Students
Piotr Chyży, MSc
Julia Macyszyn, MSc
Izabela Siekierska, MSc
Uladzislava Tsylents, MSc
Małgorzata Lobka, MSc
Helena Cichocka, MSc |
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Selected publications
- Siekierska I., Burmistrz M., Trylska J.
Evaluating delivery of peptide nucleic acids to Gram-negative bacteria using differently linked membrane-active peptides and their stapled analogs
(2024) Bioorganic and Medicinal Chemistry Letters, 114, art. no. 129993
DOI: 10.1016/j.bmcl.2024.129993
- Chyży P., Kulik M., Shinobu A., Re S., Sugita Y., Trylska J.
Molecular dynamics in multidimensional space explains how mutations affect the association path of neomycin to a riboswitch
(2024) Proceedings of the National Academy of Sciences of the United States of America, 121 (15), art. no. e2317197121
DOI: 10.1073/pnas.2317197121
- Tsylents U., Burmistrz M., Wojciechowska M., Stępień J., Maj P., Trylska J.
Iron uptake pathway of Escherichia coli as an entry route for peptide nucleic acids conjugated with a siderophore mimic
(2024) Frontiers in Microbiology, 15, art. no. 1331021
DOI: 10.3389/fmicb.2024.1331021
- Macyszyn J., Chyży P., Burmistrz M., Lobka M., Miszkiewicz J., Wojciechowska M., Trylska J.
Structural dynamics influences the antibacterial activity of a cell-penetrating peptide (KFF)3K
(2023) Scientific Reports, 13 (1), art. no. 14826
DOI: 10.1038/s41598-023-38745-y
- Dyzma A., Wielgus-Kutrowska B., Girstun A., Matošević Z.J., Staroń K., Bertoša B., Trylska J., Bzowska A.
Trimeric Architecture Ensures the Stability and Biological Activity of the Calf Purine Nucleoside Phosphorylase: In Silico and In Vitro Studies of Monomeric and Trimeric Forms of the Enzyme
(2023) International Journal of Molecular Sciences, 24 (3), art. no. 2157
DOI: 10.3390/ijms24032157
- Macyszyn J., Burmistrz M., Mieczkowski A., Wojciechowska M., Trylska J.
Conjugates of Aminoglycosides with Stapled Peptides as a Way to Target Antibiotic-Resistant Bacteria
(2023) ACS Omega, 8 (21), pp. 19047 – 19056
DOI: 10.1021/acsomega.3c02071
- Ostrowska N., Feig M., Trylska J.
Varying molecular interactions explain aspects of crowder-dependent enzyme function of a viral protease
(2023) PLoS Computational Biology, 19 (4), art. no. e1011054
DOI: 10.1371/journal.pcbi.1011054
- Tsylents U., Siekierska I., Trylska J.
Peptide nucleic acid conjugates and their antimicrobial applications—a mini-review
(2023) European Biophysics Journal, 52 (6-7), pp. 533 – 544
DOI: 10.1007/s00249-023-01673-w
- Wojciechowka, M., Macyszyn, J., Miszkiewicz, J., Grzela, R., Trylska, J.
Stapled Anoplin as an Antibacterial Agent
(2021) Front. Microbiol., 13 December
DOI: 10.3389/fmicb.2021.772038
- Chrabąszczewska, M.; Winiewska-Szajewska, M.; Ostrowska, N.; Bojarska, E.; Stępiński, J.; Mancewicz, Ł.; Łukaszewicz, M.; Trylska, J.; Taube, M.; Kozak, M.; et al.
Insight into the Binding and Hydrolytic Preferences of hNudt16 Based on Nucleotide Diphosphate Substrates.
(2021) Int. J. Mol. Sci. 2021, 22, 10929
DOI: 10.3390/ijms222010929
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