CeNT
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Cryomicroscopy and Electron Diffraction Core Facility

Tomasz Góral, PhD - experiment design consultant, microscope operator and data analyst

Research qualifications and technical skills:

  • Expertise in sample preparation for cryo-EM including glow discharging, plunge freezing and autogrid clipping

  • Fluency in operation of the 200kV cryo-TEM Glacios including EPU and TOMO software packages

  • Fluency in operation and calibration of a range of scanning electron microscopes: Zeiss Ultra Plus, FEI QUANTA 650 (ThermoFisher brand), LEO 1455VP, Phillips XL30, Zeiss EVO 15LS and a transmission electron microscope Hitachi 7100

  • Fluency in EDS analysis of a range of different samples both biological and mineralogical

  • Fluency in sample preparation techniques for SEM including dehydration and critical point drying. Experience in using high resolution sputter coaters

  • Confocal microscopy with expertise in fluorescence recovery after photobleaching (FRAP) technique, working knowledge of fluorescence lifetime imaging (FLIM) technique and total internal reflection (TIRF) microscopy. Fluency in operation of the Nikon A1- Si and Zeiss LSM700 confocal microscopes

  • Confocal image processing including signal deconvolution

  • Working knowledge of X-ray nano-computed tomography (XuM) and micro-CT imaging

  • Working knowledge of the Alicona Infinite Focus microscope

  • Operational knowledge of several imaging softwares: NIS-Elements (Nikon), Zen (Zeiss), LAS (Leica), Volocity 3D Image Analysis (Perkin Elmer), Imaris (Bitplane), Image Pro (Media Cybernetics), Volume exploration and presentation tool – Drishti, MeX (Alicona), ImageJ




Cryo-EM Core Facility at CeNT UW features:
1. Glacios 200kV cryo-EM microscope equipped with:

  • Schottky field emission source

  • Ceta 16M 200kV CCD camera

  • Falcon 3EC 200kV direct electron detection camera

  • Phase plate solution for enhanced contrast

  • Autoloader holding up to 12 grids in a cassette

  • TEM tomography data acquisition software

  • EPU software for single particle analysis experiments

2. Vitrobot for automatic vitrification of biological samples

3. Glow discharge unit (PELCO easiGlow, Ted Pella) for glow discharging TEM grids

4. IT infrastructure for data storage & analysis including storage infrastructure, servers, computational clusters, software

Cryomicroscopy and Electron Diffraction Core Facility access rates
Service typeUnassisted useAssisted use
Sample preparation (price per grid)
Plunge freezing of Quantifoil grids with Vitrobot37,00 PLN87,50 PLN
Plunge freezing of Lacey Carbon grids with Vitrobot16,50 PLN67,00 PLN
Preparation of autogrids (clipping with c-rings and c-clips)95,50 PLN146,00 PLN
Plunge freezing of Quantifoil grids + clipping130,00 PLN180,50 PLN
Plunge freezing of Lacey Carbon grids + clipping110,00 PLN160,50 PLN
Glacios microscope time:
Screening session (up to 4hrs)235,00 PLN/h*285,00 PLN/h
Full day session (8hrs) 230,00 PLN/h*280,00 PLN/h
Overnight session (24hrs)200,00 PLN/h*250,00 PLN/h
Data processing (SPA or/and cryo-ET)1,50 PLN/h52,00 PLN/h

*only for advanced users

Rates are shown here as guidance only. They refer to net values and are valid to internal users (i.e. from within the University of Warsaw).

Prices are amendable and negotiable depending on a project type, user type and a form of collaboration.

A formal acceptation of the rules and regulations is required prior to granting access to core facilities equipment and staff consultations. All information about them you can find in the form below:
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cryo-EM processing workstation
 
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Glacios 200kV cryo-TEM
 
November 2020
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Resource: Glacios 200kV cryo-TEM
Status: Approved
Eligible project/other source of financing: No dedicated source of funding
Booker: Tomasz Goral
Created: 16 Nov 2020 11:31
Modified: 16 Nov 2020 11:31
External academic and commercial users: please upload completed and signed Purchase Order in order to proceed with your reservation: null
Booker Initials/Lab Initials: TG/CF
Affiliation: CeNT - Core Facilities Staff
Internal CeNT/UW users: please upload completed and signed Internal Order Form in order to proceed with your reservation: Wycena_Zlecenia_Wewn.docx
Comments:

Service visit
Nov 20 9:00 17:00
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Resource: Glacios 200kV cryo-TEM
Status: Approved
Eligible project/other source of financing: ReMedy - P. Szwedziak
Booker: Piotr Szwedziak
Created: 24 Nov 2020 13:13
Modified: 24 Nov 2020 14:23
External academic and commercial users: please upload completed and signed Purchase Order in order to proceed with your reservation: null
Booker Initials/Lab Initials: PS
Affiliation: CeNT - Laboratory of Structural Cell Biology - Szwedziak
Internal CeNT/UW users: please upload completed and signed Internal Order Form in order to proceed with your reservation: SKonica Sec19061116251.pdf
Nov 25 9:00 17:00
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Resource: Glacios 200kV cryo-TEM
Status: Approved
Eligible project/other source of financing: External and Commercial users - please specify in the Comments Box
Booker: Tomasz Goral
Created: 13 Nov 2020 12:08
Modified: 16 Nov 2020 11:43
External academic and commercial users: please upload completed and signed Purchase Order in order to proceed with your reservation: Wycena_Zlecenia_Zewn.docx
Booker Initials/Lab Initials: TG/CF
Affiliation: External academic (please specify in the Comments box)
Internal CeNT/UW users: please upload completed and signed Internal Order Form in order to proceed with your reservation: null
Comments:

M. Czarnocki - Cieciura
Z/CED/CF/17/2020
Nov 26 9:00 17:00
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Internal Order Form to be completed and signed by non-ReMedy internal CeNT/UW users prior to making reservation assignments.

Internal User Order Form

 

External User Order Form to be completed and signed by external academic or external non-academic users prior to making reservation assignments.

External User Order Form

To reserve your consultation time, please complete the form below:

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