CeNT
herb

Transmission Electron Microscopy Core Facility

Tomasz Góral, PhD - experiment design consultant, microscope operator and data analyst

Research qualifications and technical skills:

  • Fluency in operation and calibration of a range of scanning electron microscopes: Zeiss Ultra Plus, FEI QUANTA 650 (ThermoFisher brand), LEO 1455VP, Phillips XL30, Zeiss EVO 15LS and a transmission electron microscope Hitachi 7100

  • Fluency in EDS analysis of a range of different samples both biological and mineralogical

  • Fluency in sample preparation techniques for SEM including dehydration and critical point drying. Experience in using high resolution sputter coaters

  • Confocal microscopy with expertise in fluorescence recovery after photobleaching (FRAP) technique, working knowledge of fluorescence lifetime imaging (FLIM) technique and total internal reflection (TIRF) microscopy. Fluency in operation of the Nikon A1- Si and Zeiss LSM700 confocal microscopes

  • Confocal image processing including signal deconvolution

  • Working knowledge of X-ray nano-computed tomography (XuM) and micro-CT imaging

  • Working knowledge of the Alicona Infinite Focus microscope

  • Operational knowledge of several imaging softwares: NIS-Elements (Nikon), Zen (Zeiss), LAS (Leica), Volocity 3D Image Analysis (Perkin Elmer), Imaris (Bitplane), Image Pro (Media Cybernetics), Volume exploration and presentation tool – Drishti, MeX (Alicona), ImageJ


Cryo-EM Core Facility at CeNT UW features:
1. Glacios 200kV cryo-EM microscope equipped with:

  • Schottky field emission source

  • Ceta 16M 200kV CCD camera

  • Falcon 3EC 200kV direct electron detection camera

  • Phase plate solution for enhanced contrast

  • Autoloader holding up to 12 grids in a cassette

  • TEM tomography data acquisition software

  • EPU software for single particle analysis experiments

2. Vitrobot for automatic vitrification of biological samples

3. Glow discharge unit (PELCO easiGlow, Ted Pella) for glow discharging TEM grids

4. IT infrastructure for data storage & analysis including storage infrastructure, servers, computational clusters, software

Types of sessions available:
half day (4 hrs)
full day (8 hrs)full day and overnight (24 hrs)
ReMedy Users0 PLN0 €0 $0 PLN0 €0 $0 PLN0 €0 $
Internal users: CeNT & UW 1260PLN291€321$2520 PLN582€642$7560 PLN1744€1925$
External academic users 1260PLN291€321$2520 PLN582€642$7560 PLN1744€1925$
External non-academic users 1400PLN323€357$2800 PLN646€713$8400 PLN1938€2139$
Note: For external users, VAT will be added to the amount specified

A formal acceptation of the rules and regulations is required prior to granting access to core facilities equipment and staff consultations. All information about them you can find in the form below:

cryo-EM processing workstation
 
September 2020
Mon Tue Wed Thu Fri Sat Sun
 
1
 
2
 
3
 
4
 
5
 
6
 
7
 
8
 
9
 
10
 
11
 
12
 
13
 
14
 
15
 
16
 
17
 
18
 
19
 
20
 
21
 
22
 
23
 
24
 
25
 
26
 
27
 
28
 
29
 
30
 		  
Glacios 200kV cryo-TEM
 
September 2020
Mon Tue Wed Thu Fri Sat Sun
 
1
 
2
 
3
 
4
 
5
 
6
 
7
 
8
 
9
 
10
 
11
 
12
 
13
 
14
 
15
 
16
 
17
 
18
 
19
 
20
 
21
 
22
 
23
Resource: Glacios 200kV cryo-TEM
Status: Approved
Eligible project/other source of financing: ReMedy - P. Szwedziak
Booker: Piotr Szwedziak
Created: 19 Sep 2020 11:37
Modified: 21 Sep 2020 12:01
External academic and commercial users: please upload completed and signed Purchase Order in order to proceed with your reservation: null
Booker Initials/Lab Initials: PS
Affiliation: CeNT - Laboratory of Structural Cell Biology - Szwedziak
Internal CeNT/UW users: please upload completed and signed Internal Order Form in order to proceed with your reservation: SKonica Sec19061116251.pdf
Sep 23 to Sep 25
24
Resource: Glacios 200kV cryo-TEM
Status: Approved
Eligible project/other source of financing: ReMedy - P. Szwedziak
Booker: Piotr Szwedziak
Created: 19 Sep 2020 11:37
Modified: 21 Sep 2020 12:01
External academic and commercial users: please upload completed and signed Purchase Order in order to proceed with your reservation: null
Booker Initials/Lab Initials: PS
Affiliation: CeNT - Laboratory of Structural Cell Biology - Szwedziak
Internal CeNT/UW users: please upload completed and signed Internal Order Form in order to proceed with your reservation: SKonica Sec19061116251.pdf
Sep 23 to Sep 25
25
Resource: Glacios 200kV cryo-TEM
Status: Approved
Eligible project/other source of financing: ReMedy - P. Szwedziak
Booker: Piotr Szwedziak
Created: 19 Sep 2020 11:37
Modified: 21 Sep 2020 12:01
External academic and commercial users: please upload completed and signed Purchase Order in order to proceed with your reservation: null
Booker Initials/Lab Initials: PS
Affiliation: CeNT - Laboratory of Structural Cell Biology - Szwedziak
Internal CeNT/UW users: please upload completed and signed Internal Order Form in order to proceed with your reservation: SKonica Sec19061116251.pdf
Sep 23 to Sep 25
Resource: Glacios 200kV cryo-TEM
Status: Approved
Eligible project/other source of financing: No dedicated source of funding
Booker: Tomasz Goral
Created: 11 Sep 2020 08:54
Modified: 11 Sep 2020 08:54
External academic and commercial users: please upload completed and signed Purchase Order in order to proceed with your reservation: null
Booker Initials/Lab Initials: TG/CED
Affiliation: Internal UW (other than CeNT – please specify in the Comments box)
Internal CeNT/UW users: please upload completed and signed Internal Order Form in order to proceed with your reservation: WCFCEM122020.docx
Comments:

Marcin Ziemniak - Laboratorium Badań Strukturalnych i Biochemicznych CNBCh
Sep 25 9:00 13:00
26
 
27
 
28
 
29
Resource: Glacios 200kV cryo-TEM
Status: Approved
Eligible project/other source of financing: External and Commercial users - please specify in the Comments Box
Booker: Tomasz Goral
Created: 10 Sep 2020 09:53
Modified: 10 Sep 2020 09:53
External academic and commercial users: please upload completed and signed Purchase Order in order to proceed with your reservation: Cryo_EM_dokumenty_WUM.pdf
Booker Initials/Lab Initials: TG/CED
Affiliation: External academic (please specify in the Comments box)
Internal CeNT/UW users: please upload completed and signed Internal Order Form in order to proceed with your reservation: null
Comments:

M.Szczepanski - WUM
Sep 29 10:00 14:00
30
Resource: Glacios 200kV cryo-TEM
Status: Approved
Eligible project/other source of financing: External and Commercial users - please specify in the Comments Box
Booker: Tomasz Goral
Created: 10 Sep 2020 09:53
Modified: 10 Sep 2020 09:53
External academic and commercial users: please upload completed and signed Purchase Order in order to proceed with your reservation: Cryo_EM_dokumenty_WUM.pdf
Booker Initials/Lab Initials: TG/CED
Affiliation: External academic (please specify in the Comments box)
Internal CeNT/UW users: please upload completed and signed Internal Order Form in order to proceed with your reservation: null
Comments:

M.Szczepanski - WUM
Sep 30 10:00 14:00
  

Internal Order Form to be completed and signed by non-ReMedy internal CeNT/UW users prior to making reservation assignments.

Internal User Order Form

 

External User Order Form to be completed and signed by external academic or external non-academic users prior to making reservation assignments.

External User Order Form

To reserve your consultation time, please complete the form below:

Visits: 356