The Centre of New Technologies invites to a seminar by
Paulina Kasperkiewicz-Wasilewska, PhD
Faculty of Chemistry, Wrocław University of Science and Technology,
Title: New Methods For Imaging Serine Proteases Involved In Cell Death Induction
Venue: Lecture Hall 0142 (ground floor) at 12 p.m.
Host: Prof. Joanna Trylska
Growing interest in NK and CTLs in target killing has made it imperative to develop new tools to measure the early events in pathogen elimination and targeted cell death. So far selective and potent functional small chemical tools for granzymes had not been discovered and only antibody related techniques are used in Grzs investigation, however they label total protein amount and cannot distinguish between active and inactive form of enzyme.
To overcome these limitations, we have developed innovative Grzs chemical tools that can be used to find answers for important, questioned functions of Grzs, which still remain controversial. These tools were used for imaging of Granzyme A and Granzyme B, enzymes involved in cell death induction, monitoring of their location and changes upon stimulation.