Genomics Core Facility – equipment

The very high-throughput, the most cost-effective and flexible sequencer in Illumina portfolio.

• High scalability:
  • 65-3000 Gb/run
  • 800M-10B reads/run
  • up to 2×250 bp read length
• Very high quality (typically ≥ 95% of bases higher than Q37 at 2×100 bp)
• High speed of sequencing: 13-48 h
• Can run two flow cells in parallel at once

The mid-throughput sequencer for a wide range of application.

• Scalability:
  • 20-120 Gb/run 130-400M
  • reads/run
  • up to 2×150 bp read length
• High quality (typically ≥ 90% of bases higher than Q30 at 2×75bp)
• High speed of sequencing: 11-29 h

The relatively low-throughput sequencer for small projects, amplicon or test sequencing runs.

• High scalability of low-throughput runs:
  • 0.3-15 Gb/run
  • 1-25M reads/run
  • The longest read length: up to 2×300 bp read length
• High speed of sequencing: 6-56 h

Automatic pipetting station for reaction set up.

• Two types of robotic arm: 8 – tip pipetting arm (LiHA) and labware movement arm (ROMA)
• Scalability with multiple format and size of robotic carriers – from 384-well plates to reservoirs for hundreds of ml
• Wide range of modules for liquid manipulation and carrying out reactions
  • Heating system
  • Fluorometric system
  • Magnetic separation module

Nucleic acid and proteins quality control automated system based on electrophoresis on tapes

• High sensitivity – from 10 pg for DNA , 200 pg for RNA, 5 ng for protein
• High resolution – up to 5 bp for DNA/RNA and 0.5 kDa for protein
• Broad range of different reagents for:
  • DNA (from 30bp to genomic)
  • total RNA and mRNA
  • protein (up to 200 kDa)

• High flexibility  – from one sample at a time

Nucleic acid quality control automated system based on electrophoresis on chips

• Very high sensitivity – from 5 pg for DNA , 50 pg for RNA, 0.3 ng for protein
• High resolution – up to 5 bp for DNA/RNA and 0.5 kDa for protein
• Broad range of different reagents for:
  • DNA (from 30 bp to 10 kbp)
  • total RNA and mRNA
  • small RNA
  • protein (up to 250 kDa)
• 11-12 samples at a time

Real-time PCR system

• High-throughput system – 384-well plate format
• Nucleic acids quantity and quality assessment for:
  • relative expression analysis
  • genotyping
  • sequence specific detection
  • PCR amplification optimization
  • sequencing libraries relative concentration measurements

Ultra-focused, very precise sonication system with cooling

• Very high repeatability assured by:
  • ultra-focused sound wave
  • external cooling system
  • water degassing
  • vessel construction
• High precision of very equal physical fragmentation to particular size of DNA
• High efficiency of cell disruption and chromatin fragmentation

Ultra-focused, very precise sonication system

• Very high repeatability assured by:
  • ultra-focused sound wave
  • internal temperature control system
  • vessel construction
• High precision of very equal physical fragmentation to particular size of DNA
• High efficiency of cell disruption and chromatin fragmentation