13th May 2022 (WEBINAR) Salvatore Spicuglia, Aix-Marseille University

event date: 13 May 2022

The Centre of New Technologies invites to a webinar by

Salvatore Spicuglia

Aix-Marseille University, INSERM, TAGC, UMR 1090, Marseille, France.

Title: Dissection of cis-regulatory elements with dual promoter and enhancer function: From high-throughput approaches to mechanistic insights

Date:  13th May 2022, Friday

Time: 1:00 pm (Central European Time)

Host: Prof. Dariusz Plewczyński

Room: 01.130

Zoom link: https://us02web.zoom.us/j/82426581314

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Gene expression is controlled by the involvement of gene-proximal (promoters) and distal (enhancers) regulatory elements. Our previous results have demonstrated that a subset of gene promoters, termed Epromoters, works as bona fide enhancers and regulates distal gene expression. Here, we hypothesized that Epromoters play a key role in the coordination of rapid gene induction during the inflammatory response. Using a high-throughput reporter assay we explored the function of Epromoters in response to type I interferon. We found that clusters of IFNa-induced genes are frequently associated with Epromoters and that these regulatory elements preferentially recruit the STAT1/2 and IRF transcription factors and distally regulate the activation of interferon-response genes. Based on these findings, we developed a bioinformatic pipeline to predict Epromoter-like regulated clusters. Using this pipeline, we identified and validated the involvement of Epromoter-containing clusters in the regulation of several inflammatory and stress responses. Our findings suggest that Epromoters function as a local hub recruiting the key TFs required for coordinated regulation of a subset of gene clusters during the inflammatory and stress responses.